Nicotine content of tobacco leaf estimated by UV spectrum

The spectral data of different grades flue-cured tobacco sample have been determined by UV spectrometer. Their differences were compared. The spectral prediction models of nicotine content were established through correlation analysis between the content of nicotine and spectral data of flue-cured tobacco with the stepwise multivariate regression method. The statistical results showed that the nicotine content of flue-cured tobacco were very significantly correlated to their UV spectrum. There were also very significantly correlations between the predicted and experimental values. This indicates that it would be feasible to estimate the nicotine content in flue-cured tobacco by UV spectroscopy and UV spectroscopy can be used to determine conveniently and quickly nicotine content of flue-cured tobacco leave samples without pollution.