2018Comunicata ScientiaeOpen access

Concentrations of 6-Benzylaminopurine (BAP) in micropropagation of banana ‘Farta Velhaco’ (AAB)

Gustavo Alves Pereira, Marcilio Broboff Santaella, Lefayete Michele Santana Montenegro Alves, ELDER CASSIMIRO DA SILVA, Aparecida Islayne Silva Flenga, Danilo Marcelo Aires dos Santos

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Abstract

Propagation methods such as in vitro micropropagation have been developed and improved to increase the multiplication rate in a short time and improve the seedlings quality. The objective of this study was to evaluate the efficiency of the different BAP concentrations on micropropagation of ‘Farta Velhaco’ banana during in vitro multiplication phase. The treatments were: T1 – control (0 mg L-1); T2 - 0.5 mg L-1; T3 - 1 mg L-1; T4 - 1.5 mg L-1; T5 - 2 mg L-1; T6 - 2.5 mg L-1; T7 - 3.0 mg L-1; T8 - 3.5 mg L-1 and T9 - 4.0 mg L-1 of BAP added to the MS culture medium. The total number of shoots produced per explant initial subculture every 30 days was evaluated. The highest number of shoots is recorded 2.5 mg L-1 BAP concentration, which promotes 3 shoots per explant in the 4th subculture. The 4.0 mg L-1 BAP concentration reduces the number of shoots.

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Propagation methods such as in vitro micropropagation have been developed and improved to increase the multiplication rate in a short time and improve the seedlings quality. The objective of this study was to evaluate the efficiency of the different BAP concentrations on micropropagation of ‘Farta Velhaco’ banana during in vitro multiplication phase. The treatments were: T1 – control (0 mg L-1); T2 - 0.5 mg L-1; T3 - 1 mg L-1; T4 - 1.5 mg L-1; T5 - 2 mg L-1; T6 - 2.5 mg L-1; T7 - 3.0 mg L-1; T8 - 3.5 mg L-1 and T9 - 4.0 mg L-1 of BAP added to the MS culture medium. The total number of shoots produced per explant initial subculture every 30 days was evaluated. The highest number of shoots is recorded 2.5 mg L-1 BAP concentration, which promotes 3 shoots per explant in the 4th subculture. The 4.0 mg L-1 BAP concentration reduces the number of shoots.

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Available abstract

Propagation methods such as in vitro micropropagation have been developed and improved to increase the multiplication rate in a short time and improve the seedlings quality. The objective of this study was to evaluate the efficiency of the different BAP concentrations on micropropagation of ‘Farta Velhaco’ banana during in vitro multiplication phase. The treatments were: T1 – control (0 mg L-1); T2 - 0.5 mg L-1; T3 - 1 mg L-1; T4 - 1.5 mg L-1; T5 - 2 mg L-1; T6 - 2.5 mg L-1; T7 - 3.0 mg L-1; T8 - 3.5 mg L-1 and T9 - 4.0 mg L-1 of BAP added to the MS culture medium. The total number of shoots produced per explant initial subculture every 30 days was evaluated. The highest number of shoots is recorded 2.5 mg L-1 BAP concentration, which promotes 3 shoots per explant in the 4th subculture. The 4.0 mg L-1 BAP concentration reduces the number of shoots.

Key concepts: Subculture (biology), Micropropagation, Explant culture, Shoot, Horticulture, Botany, In vitro, Biology

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Concentrations of 6-Benzylaminopurine (BAP) in micropropagation of banana ‘Farta Velhaco’ (AAB) — Research Paper | ScholarLens