Study on Extraction of Polysaccharides from Flos Hibiscus and Its Antioxidation Activity

Polysaccharides were extracted from Flos Hibiscus by hot water extraction technique. Based on the results of single-factor experiments, the effects of extraction temperature, extraction time, mass ratio of solvent to material and extraction times on the extraction yield of polysaccharides were investigated by orthogonal experiment. The clearance effect of polysaccharides from Flos Hibiscus on hydroxyl free radical ( • OH) and (O2-) superoxide anion radicals was investigated. Design as follows: extraction temperature 80° C, extraction time 3h, ratio of solvent to material 30:1ml/g. Under such conditions, the extraction yield of polysaccharide was 5.22%. The results also showed that the order of factor affecting the extraction yield of polysaccharide was extraction temperature > extraction time > ratio of solvent to material. The polysaccharides from Flos Hibiscus had strong scavenging activity against hydroxyl free radicals and superoxide radicals.