2018•Journal of Chemical EducationRequires access

Teaching Undergraduates LC–MS/MS Theory and Operation via Multiple Reaction Monitoring (MRM) Method Development

Thomas A. Betts, Julie A. Palkendo

Abstract

This laboratory experiment employs an innovative approach to introduce undergraduates to liquid chromatography–tandem mass spectrometry (LC–MS/MS) by developing a method based on multiple reaction monitoring (MRM). During this qualitative investigation, students operated a triple quadrupole mass spectrometer in the MS scan and product ion scan modes to determine precursor and product ions, respectively, for a vanillin, ethyl vanillin, and coumarin standard mixture. After identifying precursor → product ion transitions, students conducted a 2 min liquid chromatography run in MRM mode. Students were astonished by the speed, selectivity, and sensitivity of the LC–MS/MS method compared to that of a liquid chromatograph with ultraviolet detection.

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Available abstract

Key concepts: Selected reaction monitoring, Chemistry, Triple quadrupole mass spectrometer, Chromatography, Vanillin, Mass spectrometry, Liquid chromatography–mass spectrometry, Analytical Chemistry (journal)