Chloroform cometabolism by bacterial strains grown on butane
Stefano Fedi, Youry Pii, Dario Frascari, Arianna Zannoni, Massimo Nocentini, Davide Zannoni
Abstract
Stefano Fedi, Youry Pii, Dario Frascari, Arianna Zannoni, Massimo Nocentini, Davide Zannoni
Abstract
In this microcosm study we have monitored the performances of 12 butane-utilizing consortia during a long-term aerobic cometabolic degradation of chloroform (CF). After approximately 100 days of continuous CF degradation, a sudden improvement of the biodegradative performances (7-fold increase of https://www.w3.org/1998/Math/MathML" display="inline"> r c and 4-fold increase of https://www.w3.org/1998/Math/MathML" display="inline"> r c / r s ratio) was observed in two microcosms in which the highest amount of depleted CF ( https://www.w3.org/1998/Math/MathML" display="inline"> > 70 m g k g dry soil - 1 ) was observed. Two bacterial strains, isolated from the CF-degrading microcosms, were identified belonging to the genera Rhodococcus and Stenotrophomonas , respectively. A strain, named F, was further characterized to establish the involvement of the butane-monooxygenase in CF degradation.
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In this microcosm study we have monitored the performances of 12 butane-utilizing consortia during a long-term aerobic cometabolic degradation of chloroform (CF). After approximately 100 days of continuous CF degradation, a sudden improvement of the biodegradative performances (7-fold increase of https://www.w3.org/1998/Math/MathML" display="inline"> r c and 4-fold increase of https://www.w3.org/1998/Math/MathML" display="inline"> r c / r s ratio) was observed in two microcosms in which the highest amount of depleted CF ( https://www.w3.org/1998/Math/MathML" display="inline"> > 70 m g k g dry soil - 1 ) was observed. Two bacterial strains, isolated from the CF-degrading microcosms, were identified belonging to the genera Rhodococcus and Stenotrophomonas , respectively. A strain, named F, was further characterized to establish the involvement of the butane-monooxygenase in CF degradation.
Key concepts: Cometabolism, Butane, Chloroform, Chemistry, Microbiology, Bacteria, Biology, Chromatography