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2011•Unpublished venueOpen access

Regulation of Kinesin-3 activity by active zone protein SYD-2

Sailaja Mandalapu

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Abstract

This work focusses on characterizing the effect of SYD-2/kinesin interaction on the motility and mechanochemical properties of UNC-104 motor. The motility properties of molecular motors can be best studied in vitro by using recombinant motors fused to fluorescent proteins. We used fluorescent tagged motor fragment to test the effect of SYD-2 on UNC-104. Microtubule gliding assay and single molecule analysis by TIRF microscopy are well established techniques to study the motile properties of motors like velocity and processivity. In this study we aim to gain a more clear understanding of the importance of SYD-2 and UNC-104 interaction.

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What this paper is about

This work focusses on characterizing the effect of SYD-2/kinesin interaction on the motility and mechanochemical properties of UNC-104 motor. The motility properties of molecular motors can be best studied in vitro by using recombinant motors fused to fluorescent proteins. We used fluorescent tagged motor fragment to test the effect of SYD-2 on UNC-104. Microtubule gliding assay and single molecule analysis by TIRF microscopy are well established techniques to study the motile properties of motors like velocity and processivity. In this study we aim to gain a more clear understanding of the importance of SYD-2 and UNC-104 interaction.

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Available abstract

This work focusses on characterizing the effect of SYD-2/kinesin interaction on the motility and mechanochemical properties of UNC-104 motor. The motility properties of molecular motors can be best studied in vitro by using recombinant motors fused to fluorescent proteins. We used fluorescent tagged motor fragment to test the effect of SYD-2 on UNC-104. Microtubule gliding assay and single molecule analysis by TIRF microscopy are well established techniques to study the motile properties of motors like velocity and processivity. In this study we aim to gain a more clear understanding of the importance of SYD-2 and UNC-104 interaction.

Key concepts: Processivity, Kinesin, Motility, Molecular motor, Motor protein, Microtubule, Cell biology, Biophysics

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