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Sperm motility characteristics assessed by the computer - aided sperm analysis (CASA) and fertilization rates in vitro

Hrvoje Vlašić, Ivan Vlastelić, Ljiljana Randić, Neda Smiljan Severinski, Tamara Tramišak Milaković, Dejan Ljiljak, Anđelka Radojčić Badovinac

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Abstract

This study was carried out to investigate the relationships between computer-aided sperm analysis (CASA), especially the concentration and movement characteristics of progressively motile spermatozoa and fertilization rates in vitro. Semen quality analisys was performed using CASA in 69 IVF-ET cycles with at least 2 oocytes collected. Sperm quality before and after swim-up procedure was compared between 33 cycles with fertilization rate >50%, 23 cycles ≤ 50% and 13 cycles without fertilization. Our study demonstrated that velocity parameters and motility characteristics of sperm from freshly obtained semen and those after swim up procedure correlated with fertilization rates, indicating that these parameters could be good predictors of fertilization outcome.

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What this paper is about

This study was carried out to investigate the relationships between computer-aided sperm analysis (CASA), especially the concentration and movement characteristics of progressively motile spermatozoa and fertilization rates in vitro. Semen quality analisys was performed using CASA in 69 IVF-ET cycles with at least 2 oocytes collected. Sperm quality before and after swim-up procedure was compared between 33 cycles with fertilization rate >50%, 23 cycles ≤ 50% and 13 cycles without fertilization. Our study demonstrated that velocity parameters and motility characteristics of sperm from freshly obtained semen and those after swim up procedure correlated with fertilization rates, indicating that these parameters could be good predictors of fertilization outcome.

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Available abstract

This study was carried out to investigate the relationships between computer-aided sperm analysis (CASA), especially the concentration and movement characteristics of progressively motile spermatozoa and fertilization rates in vitro. Semen quality analisys was performed using CASA in 69 IVF-ET cycles with at least 2 oocytes collected. Sperm quality before and after swim-up procedure was compared between 33 cycles with fertilization rate >50%, 23 cycles ≤ 50% and 13 cycles without fertilization. Our study demonstrated that velocity parameters and motility characteristics of sperm from freshly obtained semen and those after swim up procedure correlated with fertilization rates, indicating that these parameters could be good predictors of fertilization outcome.

Key concepts: Human fertilization, Sperm, Andrology, In vitro fertilisation, Semen, Sperm motility, Biology, Motility

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