Callus induction of leaf expalnts of Talinum paniculatum

Talinum paniculatum is an edible plant with enormous medicinal properties.The main focus of this study was on callus induction due to the natural ability of callus that can regenerate into a new plant.This study was carried out to determine the effects of 2,4-Dichlorophenoxyacetic Acid (2,4-D) and 6-benzylaminopurine (BAP) on callus induction of T. paniculatum from leaf explant.Leaf explants of T. paniculatum were cultured on Murashige & Skoog (MS) media containing 2,4-D at concentrations of 1.0, 2.0, 3.0, 4.0 and 5.0 mg/L and BAP at concentrations of 0.5, 1.0, 1.5, 2.0 and 2.5 mg/L under single exposure or combined exposure.Explants cultured on medium containing no plant growth regulators (PGR) served as control in this experiment.The effectiveness of PGRs on callus induction of T. paniculatum was determined by recording the callus induction day, percentage of callus induced in all the treatments and morphology of the callus induced.Leaf explants treated with 2,4-D and BAP in combined exposure resulted in callus induction.Highest percentage of callus from leaf explants was produced on the medium containing 2.0 mg/L of 2,4-D and 2.5 mg/L of BAP in combined exposure.In conclusion, callus induction of T. paniculatum could serve as an alternative regeneration pathway for this important medicinal pant.