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2018•International Journal of Current Microbiology and Applied SciencesOpen access

Isolation and Insecticidal Potential of Native Bacillus thuringiensis against Helicoverpa armigera and Spodoptera litura

Ankit S. Patel, Harsha N. Shelat, Hiren K. Patel

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Abstract

The present investigation was carried out with an aim to isolate and characterize native Bacillus thuringiensis from Anand district of Middle Gujarat. Total 67 probable B. thuringiensis were isolated from 17 diverse soil samples, out of which 9 isolates were selected based on presence of parasporal crystal body and primary toxicity test. Selected nine native isolates were characterized based on their morpho-cultural and biochemical characteristics. Among the selected nine isolates, Abt10 isolated from soil of Navli village, Anand found the most potent. Systemic laboratory bioassay of isolate ABt-10 shown LD50 value of 2.15 X107 spores/ml and 3.7 X 106 spores/ml with the highest mortality (69.53 %) on Helicoverpa armigera and Spodoptera litura, respectively.

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The present investigation was carried out with an aim to isolate and characterize native Bacillus thuringiensis from Anand district of Middle Gujarat. Total 67 probable B. thuringiensis were isolated from 17 diverse soil samples, out of which 9 isolates were selected based on presence of parasporal crystal body and primary toxicity test. Selected nine native isolates were characterized based on their morpho-cultural and biochemical characteristics. Among the selected nine isolates, Abt10 isolated from soil of Navli village, Anand found the most potent. Systemic laboratory bioassay of isolate ABt-10 shown LD50 value of 2.15 X107 spores/ml and 3.7 X 106 spores/ml with the highest mortality (69.53 %) on Helicoverpa armigera and Spodoptera litura, respectively.

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Available abstract

The present investigation was carried out with an aim to isolate and characterize native Bacillus thuringiensis from Anand district of Middle Gujarat. Total 67 probable B. thuringiensis were isolated from 17 diverse soil samples, out of which 9 isolates were selected based on presence of parasporal crystal body and primary toxicity test. Selected nine native isolates were characterized based on their morpho-cultural and biochemical characteristics. Among the selected nine isolates, Abt10 isolated from soil of Navli village, Anand found the most potent. Systemic laboratory bioassay of isolate ABt-10 shown LD50 value of 2.15 X107 spores/ml and 3.7 X 106 spores/ml with the highest mortality (69.53 %) on Helicoverpa armigera and Spodoptera litura, respectively.

Key concepts: Spodoptera litura, Helicoverpa armigera, Bacillus thuringiensis, Bioassay, Spore, Biology, Veterinary medicine, Toxicology

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Isolation and Insecticidal Potential of Native Bacillus thuringiensis against Helicoverpa armigera and Spodoptera litura — Research Paper | ScholarLens