1960•Mspace (University of Manitoba)Open access
The fractionation and study of sunflower seed proteins
Jan Berthus Lier
Open full text 0 citations
Abstract
JTBSTRACT 'Filter paper electrophoresi-s was used.to fractionate the protaroines, alburnins, gloþulins and.glutelins extraeted' from oil-free sunflotver meal of five varieties by their res- pective solvents" At least four d.ifferent proteÍn fractions were found.in each groupa The prolamine group¡ âs extracted.by a JO"I, etbanol--water solution, contained-as many as ten d.iff erent fracti ons.The globulin group was further investigated.using precipitatÍon techniques, It lr¡as found' that the ad'd'i'i;ion of gjI, eihanol has a d.ehyd.ratingeffect on the colloid.almicel-}e, grad.uatlyprecÍpitating all protej-ns" The ad'd'ition of j-ncreasing aroounts of am:noniura sulphate, on the other hand., preferentially precipitates the proteins of high mol- ecular weight, Ä CIfliI ON.JT"EÐ GEI'IIM{T S À C 3f\T0'i',trEtG jilllEll T'S Grateful aclçnor"''¡1ed-gernent is made to Ðr ' I"Ð" Lobinson for hís assÍstance and-ad'vice through'out the coì'lrse of ihis inves',,igation ancl in the preparation of the manuscripi,' Grateful aclcno¡"'¡led-gement' Ís mao-e a"l so to Ðr' T"I'I"B" Payne for his hei-pfrrl suggestions' The provision of sunfloiver seed.s,v,ihichilrrereu-sed.intheinvestigation,by'í;heCo-oper-ativeVege-l,abieOilsliníted.,Ä1-t,ona,l.',lanitoba,ivasd"eeplyappreciated" 'i¿+5L¡.iU-U' Cúli'ì'Ëi.. .l--3l-î.Tr n l n iìTTñ -¡ T r\i'Î Il'lf'.ftujJuuarulre o o ô it¡il/IE',''i CF ITIE LII:Ïiì"{TUR'E o o TTTEORY J.'Ii' PPJIüCIPLE ÛF iiE J TIïOÐ
Open-access reader