2017•Indian Journal Of NematologyRequires access

Evaluation of Southern Root-Knot Nematode Responsive Promoter Against Other Important Plant Nematodes

S. A. Kumar, Anil Kumar, Ila Joshi, Yogesh Ekanathrao Thorat, Jyoti Kumar, Anil Sirohi, Pankaj

Abstract

A Meloidogyne incognita responsive root specific promoter was isolated from Arabidopsis root, was validated against rice root-knot nematode (M. graminicola) and reniform nematode (Rotylenchulus reniformis) at 7, 14, 21, and 28 day post inoculation (dpi) using GUS assay and qRT-PCR against both the nematodes. GUS expression was evident in Arabidopsis root against the infection of both nematode, M. graminicola and R. reniformis at each time interval. These results help us to conclude that this promoter can be utilized in engineering resistance against rice root-knot nematode (M. graminicola) and reniform nematode (R. reniformis) in commercial crops.

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Key concepts: Rotylenchulus reniformis, Nematode, Meloidogyne incognita, Biology, Graminicola, Root-knot nematode, Inoculation, Horticulture