2018University of Zagreb University Computing Centre (SRCE)Open access

Morphological and Physiological Changes of Brassica oleracea Acephala Group Seedlings as Affected by Ion and Salt Stress

Gvozden Dumičić, Juan Carlos Dı́az-Pérez, Harwinder Singh Sidhu, Branimir Urlić, Smiljana Goreta Ban, Dan MacLean, Sarah W. Workman

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Abstract

Th e aim of this study was to determine the eff ect of salt stress on morphological and physiological changes of Brassica oleracea acephala group seedlings.Seedlings of kale cultivar Red Russian (RR) and collard Croatian population Konavle 2 (K2) were grown in a fl oating hydroponic system in Tift on, Georgia, USA.Seedlings were treated with seven diff erent nutrient solutions (NS).Th e control NS (EC 2 dS m -1 ) was concentrated to achieve EC 4, 6 or 8 dS m -1 .Th ree additional salt treatments included addition of NaCl solution to the control NS to get: EC 4 NaCl (2 NS + 2 NaCl), EC 6 NaCl (2 NS + 4 NaCl) and EC 8 NaCl (2 NS + 6 NaCl) dSm -1 .Leaf gas exchange parameters decreased with increased EC.Seedlings treated with EC 6 NaCl and 8 NaCl dS m -1 had the lowest leaf relative water content (less than 59%).Seedlings treated with 2 dS m -1 had the greatest (187 cm 2 ) leaf area (LA).Cultivar RR had greater LA (131 cm 2 ) than population K2 (84 cm 2 ).Increased percentage of shoot (14.1%) and root (10.4%) dry weight (DW) was recorded in seedlings treated with EC 8 dS m -1 , c. Population K2 had higher shoot (10.9%) and root (10.4%)DW percentage compared with cv.RR.In conclusion, the nutrient solution of EC 4 NaCl had negative eff ect on morphological characteristics, compared to the same solution without NaCl.Increased concentrations of NS signifi cantly aff ected the leaf thickness (SLA) of B. oleracea acephala group seedlings.Th is can be used as production tool for seedlings hardening.

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Th e aim of this study was to determine the eff ect of salt stress on morphological and physiological changes of Brassica oleracea acephala group seedlings.Seedlings of kale cultivar Red Russian (RR) and collard Croatian population Konavle 2 (K2) were grown in a fl oating hydroponic system in Tift on, Georgia, USA.Seedlings were treated with seven diff erent nutrient solutions (NS).Th e control NS (EC 2 dS m -1 ) was concentrated to achieve EC 4, 6 or 8 dS m -1 .Th ree additional salt treatments included addition of NaCl solution to the control NS to get: EC 4 NaCl (2 NS + 2 NaCl), EC 6 NaCl (2 NS + 4 NaCl) and EC 8 NaCl (2 NS + 6 NaCl) dSm -1 .Leaf gas exchange parameters decreased with increased EC.Seedlings treated with EC 6 NaCl and 8 NaCl dS m -1 had the lowest leaf relative water content (less than 59%).Seedlings treated with 2 dS m -1 had the greatest (187 cm 2 ) leaf area (LA).Cultivar RR had greater LA (131 cm 2 ) than population K2 (84 cm 2 ).Increased percentage of shoot (14.1%) and root (10.4%) dry weight (DW) was recorded in seedlings treated with EC 8 dS m -1 , c. Population K2 had higher shoot (10.9%) and root (10.4%)DW percentage compared with cv.RR.In conclusion, the nutrient solution of EC 4 NaCl had negative eff ect on morphological characteristics, compared to the same solution without NaCl.Increased concentrations of NS signifi cantly aff ected the leaf thickness (SLA) of B. oleracea acephala group seedlings.Th is can be used as production tool for seedlings hardening.

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Available abstract

Th e aim of this study was to determine the eff ect of salt stress on morphological and physiological changes of Brassica oleracea acephala group seedlings.Seedlings of kale cultivar Red Russian (RR) and collard Croatian population Konavle 2 (K2) were grown in a fl oating hydroponic system in Tift on, Georgia, USA.Seedlings were treated with seven diff erent nutrient solutions (NS).Th e control NS (EC 2 dS m -1 ) was concentrated to achieve EC 4, 6 or 8 dS m -1 .Th ree additional salt treatments included addition of NaCl solution to the control NS to get: EC 4 NaCl (2 NS + 2 NaCl), EC 6 NaCl (2 NS + 4 NaCl) and EC 8 NaCl (2 NS + 6 NaCl) dSm -1 .Leaf gas exchange parameters decreased with increased EC.Seedlings treated with EC 6 NaCl and 8 NaCl dS m -1 had the lowest leaf relative water content (less than 59%).Seedlings treated with 2 dS m -1 had the greatest (187 cm 2 ) leaf area (LA).Cultivar RR had greater LA (131 cm 2 ) than population K2 (84 cm 2 ).Increased percentage of shoot (14.1%) and root (10.4%) dry weight (DW) was recorded in seedlings treated with EC 8 dS m -1 , c. Population K2 had higher shoot (10.9%) and root (10.4%)DW percentage compared with cv.RR.In conclusion, the nutrient solution of EC 4 NaCl had negative eff ect on morphological characteristics, compared to the same solution without NaCl.Increased concentrations of NS signifi cantly aff ected the leaf thickness (SLA) of B. oleracea acephala group seedlings.Th is can be used as production tool for seedlings hardening.

Key concepts: Brassica oleracea, Salt (chemistry), Botany, Biology, Stress (linguistics), Horticulture, Brassica, Chemistry

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