2004•Zhonghua weishengwuxue he mianyixue zazhiRequires access
Expression of L1 major capsid protein from human papillomavirus type 16 and the production of virus-like particles
Lanlan Wei, GU Hong-xi, Chu Ming, Wang Yan, Zhuang Min, Qinglong Shang, Hong Ling, Fengmin Zhang
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Abstract
Objective To express the L1 major capsid protein of regional variant strain of human papillomavirus type 16, and to purify HPV16 L1 virus-like particles. Methods The recombinant plasmids including HPV16 L1 gene was constructed and the L1 protein was expressed by infected Sf9 cells. Optimizating the amplification of recombinant virus and expression level of L1 protein by changing the MOI and the infecting time. VLPs of L1 protein in Sf9 cells was analyzed by electron microscopy. VLPs were purified with CsCl gradient centrifugation. Results The stable strain of recombinant baculovirus expressing HPV16 L1 protein was obtained. These 55nm diameter particles were present in the nuclei of recombinant baculovirus-infected insect cells with density of 1.29- 1.30 /cm 3. Conclusion HPV16 L1 protein could be efficiently expressed in Sf9 cells and VLPs of HPV16 L1 protein could be purified by CsCl gradient centifugation.