2016•Royal Society of Chemistry eBooksRequires access
1.3. Isolation of Plant Pigments from Green and Red Leaves
Alice Dias, Maria La Salete Ferreira
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Abstract
This experiment describes a colourful column chromatography of both lipophilic and hydrophilic plant pigments that agrees with green chemistry principles. A solid extract of green and red plant leaves is prepared by adsorption of crude extract on a household adsorbent. Column chromatography packed with the same adsorbent separates pigment bands with the use of common eluents. Paper chromatography is used to evaluate the eluted fractions.