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2017•Unpublished venueRequires access

Relaxant Effect of Ethanolic Extract of Eriobotrya japonica Leaves on Isolated Guinea Pig Tracheal Chains

Marianne Marianne, Urip Harahap, Emil Salim, Dadang Irfan Husori, Fahrumsyah Jali Rambe

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Abstract

Objective: To examine the inhibitory effect of ethanolic extract of Eriobo t rya japonica leaves (EEEJL) on isolated guinea pig tracheal chains against acetylcholine (ACh)-induced contraction of the Ach-muscarinic-3 receptor. Materials and Methods: The effect of EEEJL (1-8 mg/ml) on the isolated tracheal strips was tested in vitro. Furthermore, mechanism of relaxant effects of EEEJL was evaluated in the presence of theophylline, a non-selective phosphodiesterase inhibitor. Results: The contractile response evoked by Ach ( 1.25.10 -3 M) was decreased by EEEJL (EC 50 =1.36 mg/ml) and has no significant different of relaxant effect to that of EEEJL pre-incubated with theophylline (p>0.05). Conclusion: The EEEJL decreased the Ach-induced contraction via the inhibition of phosphodiesterase. Keywords: Erioboryta japonica , Biwa, ACh-muscarinic-3 receptor, theophylline

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Objective: To examine the inhibitory effect of ethanolic extract of Eriobo t rya japonica leaves (EEEJL) on isolated guinea pig tracheal chains against acetylcholine (ACh)-induced contraction of the Ach-muscarinic-3 receptor. Materials and Methods: The effect of EEEJL (1-8 mg/ml) on the isolated tracheal strips was tested in vitro. Furthermore, mechanism of relaxant effects of EEEJL was evaluated in the presence of theophylline, a non-selective phosphodiesterase inhibitor. Results: The contractile response evoked by Ach ( 1.25.10 -3 M) was decreased by EEEJL (EC 50 =1.36 mg/ml) and has no significant different of relaxant effect to that of EEEJL pre-incubated with theophylline (p>0.05). Conclusion: The EEEJL decreased the Ach-induced contraction via the inhibition of phosphodiesterase. Keywords: Erioboryta japonica , Biwa, ACh-muscarinic-3 receptor, theophylline

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Available abstract

Objective: To examine the inhibitory effect of ethanolic extract of Eriobo t rya japonica leaves (EEEJL) on isolated guinea pig tracheal chains against acetylcholine (ACh)-induced contraction of the Ach-muscarinic-3 receptor. Materials and Methods: The effect of EEEJL (1-8 mg/ml) on the isolated tracheal strips was tested in vitro. Furthermore, mechanism of relaxant effects of EEEJL was evaluated in the presence of theophylline, a non-selective phosphodiesterase inhibitor. Results: The contractile response evoked by Ach ( 1.25.10 -3 M) was decreased by EEEJL (EC 50 =1.36 mg/ml) and has no significant different of relaxant effect to that of EEEJL pre-incubated with theophylline (p>0.05). Conclusion: The EEEJL decreased the Ach-induced contraction via the inhibition of phosphodiesterase. Keywords: Erioboryta japonica , Biwa, ACh-muscarinic-3 receptor, theophylline

Key concepts: Theophylline, Muscarinic acetylcholine receptor, Acetylcholine, Chemistry, Guinea pig, Contraction (grammar), Eriobotrya, Pharmacology

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