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In-vitro plantlet regeneration from shoot tip of field-grown hermaphrodite papaya (Carica papaya L. cv. Eksotika)

Syaiful Bahri Panjaitan, Aziz ur Rehman Aziz, Abdul Rashid

Open publisher page 19 citations

Abstract

An experiment was carried out on plantlet regeneration from shoot tip of hermaphrodite field-grown papaya (Carica papaya L. cv. Eksotika). BAP concentrations in combination with NAA were assessed for shoot induction and proliferation. For rooting, pretreatment in different indol-3-butyric acid (IBA) concentrations, followed by culture on medium with and without vermiculite supplement were evaluated. MS medium containing 1.0 mg L 6-benzylaminopurine (BAP) and 0.05 mg L αnaphthaleneacetic acid (NAA) produced the highest mean number of shoots (73.3) per explant. The number of emerging shoots per explant differed significantly from all other treatments including the control. A highest rooting percentage (95%) was obtained on shoots pretreated in 1.0 mg L IBA. Culture on medium supplemented with vermiculite, exhibited a mean number of 1.4 roots per shoot and mean root length of 2 cm. A protocol for in-vitro plantlet regeneration from shoot tip of hermaphrodite field-grown papaya (Carica Papaya L. cv. Eksotika) is developed.

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What this paper is about

An experiment was carried out on plantlet regeneration from shoot tip of hermaphrodite field-grown papaya (Carica papaya L. cv. Eksotika). BAP concentrations in combination with NAA were assessed for shoot induction and proliferation. For rooting, pretreatment in different indol-3-butyric acid (IBA) concentrations, followed by culture on medium with and without vermiculite supplement were evaluated. MS medium containing 1.0 mg L 6-benzylaminopurine (BAP) and 0.05 mg L αnaphthaleneacetic acid (NAA) produced the highest mean number of shoots (73.3) per explant. The number of emerging shoots per explant differed significantly from all other treatments including the control. A highest rooting percentage (95%) was obtained on shoots pretreated in 1.0 mg L IBA. Culture on medium supplemented with vermiculite, exhibited a mean number of 1.4 roots per shoot and mean root length of 2 cm. A protocol for in-vitro plantlet regeneration from shoot tip of hermaphrodite field-grown papaya (Carica Papaya L. cv. Eksotika) is developed.

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Available abstract

An experiment was carried out on plantlet regeneration from shoot tip of hermaphrodite field-grown papaya (Carica papaya L. cv. Eksotika). BAP concentrations in combination with NAA were assessed for shoot induction and proliferation. For rooting, pretreatment in different indol-3-butyric acid (IBA) concentrations, followed by culture on medium with and without vermiculite supplement were evaluated. MS medium containing 1.0 mg L 6-benzylaminopurine (BAP) and 0.05 mg L αnaphthaleneacetic acid (NAA) produced the highest mean number of shoots (73.3) per explant. The number of emerging shoots per explant differed significantly from all other treatments including the control. A highest rooting percentage (95%) was obtained on shoots pretreated in 1.0 mg L IBA. Culture on medium supplemented with vermiculite, exhibited a mean number of 1.4 roots per shoot and mean root length of 2 cm. A protocol for in-vitro plantlet regeneration from shoot tip of hermaphrodite field-grown papaya (Carica Papaya L. cv. Eksotika) is developed.

Key concepts: Carica, Plantlet, Shoot, Explant culture, Vermiculite, Horticulture, Hermaphrodite, Biology

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In-vitro plantlet regeneration from shoot tip of field-grown hermaphrodite papaya (Carica papaya L. cv. Eksotika) — Research Paper | ScholarLens