Kisspeptin, unexplained infertility and embryo implantation

The primary objective of the study was to compare baseline KP levels in females with different causes of infertility and then correlate it with the risk of failure to conceive, preclinical abortion and pregnancy after intracytoplasmic sperm injection (ICSI)..AA longitudinal cohort study was carried out from August 2014 till May 2015 recruiting one hundred and twenty-four patients of ICSI, after obtaining ethical approval from Australian Concept Infertility Medical Centre. Cause of infertility in 124 females due to male, female and unexplained causes was; 32 (24%), 33 (31%) and 59 (45%). KP levels were measured by ELISA assay before the initiation of ICSI treatment protocol. Outcome of ICSI was declared in groups; non-pregnant with beta human chorionic gonadotropin (β HCG) 25mIU/ml with no cardiac activity and clinical pregnancy declared upon confirmation of cardiac activity. Results on the basis of cause of infertility and outcome groups were compared by One Way Analysis of Variance..Unexplained infertile females had significant low levels of KP as compared to male factor infertility cause (176.69± 5.03 vs. 397.6 ± 58.2, p=0.001). Clinical pregnancy was acquired by 28 (23%) females out of which 17 (71%) had a female cause of infertility. In non-pregnant group of 66 (53%) females, common cause of infertility was unexplained 56(85%). A weak positive correlation of KP with fertilized oocytes and endometrial thickness (p=0.04 and 0.01 respectively) was observed..Deficiency of KP in females with unexplained infertility was associated with reduced chances of implantation after ICSI.