Effect of Extract Black Cumin (Nigella sativa) on the Vaginal Cytology of Ovariectomized White Mouse (Rattus Novergicus Strain Wistar)

Background: Hypoestrogen in menopausal women causes loss of glycogen, decreased lactic acid production that could rise the vaginal pH and facilitates the occurrence of infection, irritation and vaginal becomes dry, hot and itchy.The importance of the role of estrogen for women reproduction, encouraging researches to find the source of estrogen from the others.Black cumin is a spice that can be used as a medicinal plant and food preservatives.Objectives: The study aimed to determine the effect of black cumin against changes on vaginal cytology of the ovariectomized white mouse.Methods: This study was true experimental design with the posttest only control group designed with 21 female mouse divided into seven groups: a control negative group, a control positive group, and 5 treatment groups of black cumin extract at a dose of 1.25, 2.5, 5, 10 and 20 mg/kg BW/day.Results: The results result showed the significant differences in intermediate cells changes (p: 0,001), superficial cells (p: 0,000) and not significant in parabasal cell (p:0120).By the Kruskal-Wallis, there was significant difference in vaginal maturation index (p: 0,22).Conclusion: Extract of black cumin had a significant effect on the vaginal cytology of ovariectomized white rats.