Optimization of Bacteria Amylase Activity from Bacillus licheniformis Strain SEM11

Amylase produced by bacteria or bacteria amylase is an enzyme that can hydrolyze starch to sugar. Bacteria amylase is successfully replaced chemical hydrolysis in starch processing industries. Bacteria amylases are commercially important selectively hydrolyze the α-1,4 sugar linkage in polysaccharides. Bacteria amylase is used commercially in many industries such as textile, pharmaceutical, food, glucose syrup, and detergent additive. Bacteria amylases are traded as much as 25% of the total another enzyme. Bacteria amylase from thermo-alkaliphile bacteria more attractive to use in industry because it is resistant to high temperature and pH. Bacillus licheniformis strain SEM11 is an amylase-producing bacteria that has been isolated from Semurup hot spring. Semurup hot spring, Kerinci district, Jambi province, Indonesia has the temperature 80°C dan pH 8.4. Bacteria amylase activity of Bacillus licheniformis strain SEM11 still low is 68, 52 U/ml because it’s wild-type bacteria. The purpose of this study was to increase bacteria amylase activity of Bacillus licheniformis strain SEM11 through optimization of bacteria amylase activity. The results showed that bacteria amylase activity increased by using 1.5% rice substrate, at temperature 55°C, pH 8.0, fructose as the carbon source, and soybean as a source of nitrogen. Bacteria amylase from thermo-alkaliphilic Bacillus licheniformis strain SEM11 potentially in various industries because it has a high bacteria amylase activity.