Contribution of Propionibacterium Acnes Bacteria in Patients with Acne Vulgaris in Makassar

Propionibacterium acnes is a microaerophilic organism in acne lesion. The study aims to determine the relationship between the number of Propionibacterium acnes bacteria colonies and the degrees of acne vulgaris. The research was cunducted at high school in Makassar city on july 2017- august 2017 as an observational analytic study with cross sectional method. The samples were fort- four (44) acne vulgaris patients of 14 to 20 years old at high schools in Makassar city. Blackheads were taken from acne vulgaris lesions of the 44 sample. The process was followed with gram stain, anaerobic bacterial culture, and polymerase chain reaction examination ( PCR ). The  Results from the anaerobic bacteria culture examination of 44 peoples were, 33 (75%) Staphylococcus epidermididis, 1 (2,3% Staphylococcus xylosus, 4 (9%) Staphylococcus hominis, 1 (2,3%) Staphylococcus aureus, 1 (2.3%) Staphylococcus haemolyticus, 1 (2.3%) Staphylococcus capitis, 2 (4,5%) Staphylococcus warneri, 1 (2.3%) Lactobacillus plantarum and none of propionibacterium acnes. Furthermore the PCR examination showed that there was no Propionibacterium acnes on mild acne vulgaris but in five students (17,8%) had positive results of Propionibacterium acnes in moderate and severe acne.It showed that there was a statistical significance result with biological relevance, Propionibacterium acnes contribute to moderate and severe acne.