Simultaneous Determination of Protopine and Palmatine Chloride in Qingnaozhitong Capsule by HPLC

清脑止痛胶囊主要由白芍、川芎、葛根、延胡索、蒺藜、白芷、刺五加、夏天无八味药经过水蒸气蒸馏、水提、醇提三种工艺制备，具有祛风活血、止痛安神的功效。现有质量标准对君药白芍所含芍药苷进行了含量测定，并对葛根、夏天无、延胡索、刺五加的薄层进行了鉴别，方法稳定可行。白芍为水提药材，为进一步控制产品质量，本文采用高效液相色谱(HPLC)法同时测定清脑止痛胶囊中醇提部位夏天无中原阿片碱和盐酸巴马汀的含量，研究结果表明，本方法灵敏、快速、可靠，可用于清脑止痛胶囊的质量控制。 Qingnaozhitong Capsule is mainly composed with Radix Paeoniae Alba, Ligusticum wallichii, Radices Paeoniae Alba, Rhizoma Corydalis, Tribulus terrestris, Angelica dahurica, Acanthopanax Senticosus and Corydalis Amabilis which are processed by steam distillation, water extraction and alcohol extraction. They have the effect of activating blood, relieving pain and calming the nerves. The content of paeoniflorin in Radix Paeoniae Alba was determined by the existing quality standards. Radices Paeoniae Alba, Corydalis Amabilis, Rhizoma Corydalis and Acanthopanax Senticosus were identified by TLC. Paeoniflorin is from water extraction, in order to control the quality of products further. HPLC was used to determinate protopine hydrochloride and Pakistan Martin, which is extracted by ethanol from Rhizoma Corydalis. The results show that the method is sensitive, rapid and reliable, and can be used for quality control of Qingnaozhitong capsule.