Development of a SYBR green-based real-time quantitative PCR assay to detect PCV3 in pigs
Guihua Chen, Xiaoyu Tang, Yuan Sun, Ling Zhou, Di Li, Yang Bai, Kaijie Mai, Yiyun Li, Qiwen Wu, Jingyun Ma
Abstract
Guihua Chen, Xiaoyu Tang, Yuan Sun, Ling Zhou, Di Li, Yang Bai, Kaijie Mai, Yiyun Li, Qiwen Wu, Jingyun Ma
Abstract
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Key concepts: SYBR Green I, Real-time polymerase chain reaction, Biology, Molecular biology, Melting curve analysis, Coefficient of variation, Detection limit, Quantitative analysis (chemistry)