Steam explosion pretreatment and zymohydrolysis of corn cobs.
Tianbao Ren, Qiu Dong Pan, Ren Xiujuan, Zhang AnBang, Yang Bo, Dongsheng Zhang
Abstract
Tianbao Ren, Qiu Dong Pan, Ren Xiujuan, Zhang AnBang, Yang Bo, Dongsheng Zhang
Abstract
In this study,Utilizing corn cobs after steam explosion as material to research on enzymatic hydrolysis of corn cobs.The results showed,maintenance pressure time 240 s and steam pressure 2.8 MPa,the initial ratio of solid content was 14%(W/V),added cellulase(15 FPA/g,zymolyte),xylanase(225 IU/g,zymolyte) under pH 5.0 respectively.Simultaneously the environmental factors(MgSO4 0.005 g/g and Tween-80 0.001 g/g) were added.After 48 hours,the final concentration of reducing sugars was 71.81 g/L and saccharification rate reached 80.85%.This study demonstrated that the steam explosion pretreatment and proper amount of environmental factors could significantly improve the reducing sugars of corn cobs.
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In this study,Utilizing corn cobs after steam explosion as material to research on enzymatic hydrolysis of corn cobs.The results showed,maintenance pressure time 240 s and steam pressure 2.8 MPa,the initial ratio of solid content was 14%(W/V),added cellulase(15 FPA/g,zymolyte),xylanase(225 IU/g,zymolyte) under pH 5.0 respectively.Simultaneously the environmental factors(MgSO4 0.005 g/g and Tween-80 0.001 g/g) were added.After 48 hours,the final concentration of reducing sugars was 71.81 g/L and saccharification rate reached 80.85%.This study demonstrated that the steam explosion pretreatment and proper amount of environmental factors could significantly improve the reducing sugars of corn cobs.
Key concepts: Steam explosion, Cellulase, Chemistry, Xylanase, Hydrolysis, Steam pressure, Food science, Enzymatic hydrolysis