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1985•Journal of Entomological ScienceOpen access

ACTIVITY ON THE BETA-EXOTOXIN OF BACILLUS THURINGIENSIS VAR. THURINGIENSIS IN THE ESCHERICHIA COLI DNA REPAIR ASSAY FOR BACTERIAL GENOTOXICITY1,2

George E. Cantwell, Robert J. Argauer, W. W. Cantelo

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Abstract

The beta-exotoxin of an experimental preparation of Bacillus thuringiensis var. thuringiensis was bioassayed against neonate Colorado potato beetle larvae and its lethal concentrations determined. The amount of active ingredient in the preparation was determined by liquid chromatography. The Escherichia coli DNA Repair Assay was used to determine the DNA damaging potential of the beta-exotoxin, which was found to be negative in this system.

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The beta-exotoxin of an experimental preparation of Bacillus thuringiensis var. thuringiensis was bioassayed against neonate Colorado potato beetle larvae and its lethal concentrations determined. The amount of active ingredient in the preparation was determined by liquid chromatography. The Escherichia coli DNA Repair Assay was used to determine the DNA damaging potential of the beta-exotoxin, which was found to be negative in this system.

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Available abstract

The beta-exotoxin of an experimental preparation of Bacillus thuringiensis var. thuringiensis was bioassayed against neonate Colorado potato beetle larvae and its lethal concentrations determined. The amount of active ingredient in the preparation was determined by liquid chromatography. The Escherichia coli DNA Repair Assay was used to determine the DNA damaging potential of the beta-exotoxin, which was found to be negative in this system.

Key concepts: Bacillus thuringiensis, Exotoxin, Escherichia coli, Biology, Microbiology, DNA, Bacteria, Toxin

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ACTIVITY ON THE BETA-EXOTOXIN OF BACILLUS THURINGIENSIS VAR. THURINGIENSIS IN THE ESCHERICHIA COLI DNA REPAIR ASSAY FOR BACTERIAL GENOTOXICITY1,2 — Research Paper | ScholarLens