Experimental study of HA14-1-induced apoptosis of Lewis lung cancer cell.

Objective To observe the effect of HA14-1 on the apoptosis of Lewis lung cancer (LLC)cells in vitro. Methods Cultured LLC cells were divided into experimental group and control group. Different concentrations of HA14-1 (12.5,25,50,75,100 mmol/L)was added into the cells of experimental groups respectively for 24,48,72 hours. The LLC cell survival fraction,apoptotic rate and Bcl-2 expression before and after HA14-1 treatment were detected by MTT,flowcytometry and immunohistochemistry respectively. Results Compared with the blank control group,the cell survival rate of the experimental group declined with the increase of HA14-1 concentration,while the apoptotic rate increased with the concentration of HA14-1 (P 0.01);the results of immunohistochemistry showed that Bcl-2 was expressed strongly in LLC cells and HA14-1 induced a decrease of Bcl-2 expression in LLC cells. Conclusion HA14-1 may promote the apoptosis of LLC cells.