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The Determination of Fumarase and Aconitase in Rat Liver Homogenate by Means of the Modified Racker’s Method

Wojciech Antoni Turski, Jolanta Szkudlarek

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Abstract

A modification of Racker’s spectrophotometric method for the determination of fumarase (fumarate hydratase) and aconitase (aconitate hydratase) has been described. The described modification is more sensitive and more convenient than other methods. Beyond this, it may be used for the estimations in big series of samples. It also enables to obtain highest activities of both investigated enzymes at a suitable way of dilution, without the detergents. The inhibiting influence of DOC on fumarase but not on aconitase activity has been observed. Triton X-100 exerts no additional influence on the activity of both enzymes when compared with that of diluted samples without detergent.

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What this paper is about

A modification of Racker’s spectrophotometric method for the determination of fumarase (fumarate hydratase) and aconitase (aconitate hydratase) has been described. The described modification is more sensitive and more convenient than other methods. Beyond this, it may be used for the estimations in big series of samples. It also enables to obtain highest activities of both investigated enzymes at a suitable way of dilution, without the detergents. The inhibiting influence of DOC on fumarase but not on aconitase activity has been observed. Triton X-100 exerts no additional influence on the activity of both enzymes when compared with that of diluted samples without detergent.

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Available abstract

A modification of Racker’s spectrophotometric method for the determination of fumarase (fumarate hydratase) and aconitase (aconitate hydratase) has been described. The described modification is more sensitive and more convenient than other methods. Beyond this, it may be used for the estimations in big series of samples. It also enables to obtain highest activities of both investigated enzymes at a suitable way of dilution, without the detergents. The inhibiting influence of DOC on fumarase but not on aconitase activity has been observed. Triton X-100 exerts no additional influence on the activity of both enzymes when compared with that of diluted samples without detergent.

Key concepts: Fumarase, Aconitase, Chemistry, Enzyme, Biochemistry, Chromatography

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