2012•Zhonghua shiyan waike zazhiRequires access

Inhabitory effects of basic fibroblast growth factor small interfering RNA on expression of vascular endothelial growth factor in pancreatic carcinoma cells

Yan Changqing, Zhao Yu-Pei, Sanguang Liu, Qi Cheng, Jiansheng Zhang, Wenbin Wang

Abstract

Objective To investigate the effects of basic fibroblast growth factor (bFGF) small interfering RNA (siRNA) on the expression of vascular endothelial growth factor (VEGF) in pancreatic carcinoma cells.Methods The expression and secretion of VEGF and bFGF in pancreatic carcinoma cells were inhabited by VEGF siRNA and bFGF siRNA.The expression of VEGF in pancreatic carcinoma cells was detected by using reverse transcription-polymerase chain reaction (RT-PCR) and enzyme linked immunosorbent assay (ELISA).Results Both VEGF siRNA and bFGF siRNA significantly inhabited the expression of VEGF mRNA in Panc-1 and PCT-3 cells.Both VEGF siRNA and bFGF siRNA significantly inhabited the expression of VEGF in sw1990 and PCT-3 cells.Conclusion The bFGFsiRNA inhabited the expression of VEGF mRNA and VEGF in pancreatic carcinoma cells. Key words: Pancreatic carcinoma; Vascular endothelial growth factor; Basic fibroblast growth factor

About this research paper

What this paper is about

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Basic fibroblast growth factor, Vascular endothelial growth factor, Small interfering RNA, Pancreatic cancer, Cancer research, Pancreatic carcinoma, Messenger RNA, Growth factor