Rekombinogenost pseudopalindroma u genomu kvasca Saccharomyces cerevisiae

Genomes of all organisms, especially of higher eukaryotes, contain a significant proportion of repeated sequences. A special group of inverted repeats are closely spaced inverted repeats known as palindromes (perfect palindromes) and pseuodopalindromes (imperfect palindromes). A palindrome is composed of two identical inverted repeats which are not separated, whereas inverted repeats in pseudopalindromes are not identical and/or they are separated by one or more base pairs (bp). Palindromes and pseudopalindromes can form DNA secondary structures which can lead to genetic diseases in human. In this study the influence of pseudopalindromes, i.e. the distance between inverted repeats on its recombinogenicity was investigated. As experimental organism yeast Saccharomyces cerevisiae was used. The results of this study showed that the insertion of 4 bp spacer DNA in the center of a 126 bp long palindrome does not reduce its recombinogenicity, but the 10 bp spacer DNA completely abolishes recombinogenicity of 126 and 150 bp long palindromes.