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2014•Unpublished venueRequires access

Propagation of Aspergillus niger in Stirred Fermentor for the Production of Glucose Oxidase

Irshad Ahmad, Zia Ul Islam, Zhisheng Yu, Muhammad Mohsin Javed, Khyber Pakhtunkhwa

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Abstract

The present research work was planned for enhanced glucose oxidase production in Aspergillus niger by optimizing the growth conditions in a stirred fermentor. The results revealed that medium M2 containing (g/L) glucose 80.0, peptone 3.0, (NH4)2HPO4 0.388, KH2PO4 0.188 and MgSO4.7H2O 0.156 was the best among four tested media. Glucose (8%) was the best carbon source for the maximum yield of glucose oxidase (118 U/g cell mass) followed by sucrose (103 U/g cell mass) and fructose (99 U/g cell mass). (NH4)2HPO4 followed by peptone were the best nitrogen sources for the growth of A. niger and production of glucose oxidase. The maximum activity (118 U/g) of glucose oxidase was produced after 72 hours of fermentation at pH 5.0, temperature 30°C, agitation 200 rpm and aeration of 1 vvm.

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What this paper is about

The present research work was planned for enhanced glucose oxidase production in Aspergillus niger by optimizing the growth conditions in a stirred fermentor. The results revealed that medium M2 containing (g/L) glucose 80.0, peptone 3.0, (NH4)2HPO4 0.388, KH2PO4 0.188 and MgSO4.7H2O 0.156 was the best among four tested media. Glucose (8%) was the best carbon source for the maximum yield of glucose oxidase (118 U/g cell mass) followed by sucrose (103 U/g cell mass) and fructose (99 U/g cell mass). (NH4)2HPO4 followed by peptone were the best nitrogen sources for the growth of A. niger and production of glucose oxidase. The maximum activity (118 U/g) of glucose oxidase was produced after 72 hours of fermentation at pH 5.0, temperature 30°C, agitation 200 rpm and aeration of 1 vvm.

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Available abstract

The present research work was planned for enhanced glucose oxidase production in Aspergillus niger by optimizing the growth conditions in a stirred fermentor. The results revealed that medium M2 containing (g/L) glucose 80.0, peptone 3.0, (NH4)2HPO4 0.388, KH2PO4 0.188 and MgSO4.7H2O 0.156 was the best among four tested media. Glucose (8%) was the best carbon source for the maximum yield of glucose oxidase (118 U/g cell mass) followed by sucrose (103 U/g cell mass) and fructose (99 U/g cell mass). (NH4)2HPO4 followed by peptone were the best nitrogen sources for the growth of A. niger and production of glucose oxidase. The maximum activity (118 U/g) of glucose oxidase was produced after 72 hours of fermentation at pH 5.0, temperature 30°C, agitation 200 rpm and aeration of 1 vvm.

Key concepts: Aspergillus niger, Glucose oxidase, Fermentation, Sucrose, Aeration, Food science, Chemistry, Fructose

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