A HPTLC method for estimation of solasodine and diosgenin in Solanum xanthocarpum Schrad. and Wendl.

The present paper deals with development and standardization of HPTLC method used for quantification of Solasodine and Diosgenin in Solanum xanthocarpum. Solanum xanthocarpum Schrad. and Wendl. (Kantakari) Solanaceae is known inAyurveda for its activity in bronchial asthma, fungal infections, heart diseases, diabetes and a general low vitality of the system. It containsmany phyto-constituents such asDiosgenin, Solasodine and other steroidal alkaloids.As there is no single chromatographicmethod for estimation of Solasosodine and Diosgenin, which can be used for standardization of extract of Solanumxanthocarpum, an attempt has beenmade to quantify Solasodine and Diosgenin in Solanum xanthocarpum dried crude fruit and extracts by single HPTLC method. The lowest detectable limit of Solasodine and Diosgenin was found upto 10 ng and 20 ng respectively. This method also provides good resolution and separation of Solasodine and Diosgenin from other constituents of Solanum xanthocarpum Schrad. andWendl. Further, recovery values of Solasodine and Diosgenin were found to be about 92% and 93% respectively, which shows the reliability and suitability of the method. This HPTLC method was found to be reproducible, accurate and precise. The structure of isolated Solasodine and Diosgenin was characterized and confirmed by various advanced spectroscopic methods.