Morphological Properties of Mesenchymal Stem Cells Derived from Bone Marrow of Rhesus Monkeys (in English)

To establish an in vitro system for isolat ing and culturing the mesenchymal stem cells (MSC) of Rhesus monkeys, and to pro vide research data for its further application, the bone marrow of Rhesus monkey s was collected and separated by gradient centrifugation to discard most of the blood cells. The MSC contained in the monocyte centrifuging layer was obtained a nd cultured in Dulbecco's modified media (low glucose, L-DMEM) sup plemented with 10% Fetal bovine serum (FBS) and 1 ng/ml basic fibroblast growth factor (bFGF). The non-MSC was screened out by continuously renewing the mediu m. A passage culture was undertaken while the MSC monolayer formed. The spindle- shaped MSC formed a monolayer after 18 days of primary culturing, and the cell s appeared in an oriented array with a swirling and irradiating growth trend. In the anaphase of passage culture, the cell proliferation rate was decreased and the morphology changed into triangular, polygon and flat appearance. These resul ts suggested that mesenchymal stem cells (MSC) of the Rhesus monkey can be passa ged in vitro with the established optimized culture system.