GERMINATION OF CONCENTRATED SUSPENSIONS OF SPORES FROM
Javier Barrios-González
Abstract
Javier Barrios-González
Abstract
SUMMARY Germination of condiospores from A.niger in very concentrated suspension was required to inoculate solid fermentation media, but a germination self-inhibitor was detected in spores. It was found that the inhibitory effect depended on the composition of the medium and was reduced when glucose was used as a carbon source. The effect of the .self-inhibitor was eliminated by washing the spores and using glucose and a protein nitrogen source in the germination medium. By this method it was possible to increase about 100 times (106 to 108 ) spore concentration, keeping more than 90% germination.
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SUMMARY Germination of condiospores from A.niger in very concentrated suspension was required to inoculate solid fermentation media, but a germination self-inhibitor was detected in spores. It was found that the inhibitory effect depended on the composition of the medium and was reduced when glucose was used as a carbon source. The effect of the .self-inhibitor was eliminated by washing the spores and using glucose and a protein nitrogen source in the germination medium. By this method it was possible to increase about 100 times (106 to 108 ) spore concentration, keeping more than 90% germination.
Key concepts: Germination, Spore, Spore germination, Carbon source, Suspension (topology), Fermentation, Chemistry, Food science