2016Journal of Tropical Plant ProtectionOpen access

Selection In vitro of Granola Potato M1 Againts Bacteria Wilt Disease Ralstonia solanacearum

Hidayat Asta, Tatik Wardiyati, Mochamad Dawam Maghfoer

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Abstract

Abstrac Bacterial wilt disease caused by Ralstonia solanacearum is an important disease and could decreased yield up to 80%. Generally, bacterial disease is more difficult to control than other diseases. Moreover, there are no chemicals that can be used to control this pathogen. The approach through genetic improvement by using Ethyl Methane Sulfonate and in vitro selection are procesess to generate variants resistant against bacterial wilt on potato plants. Granola potato M 1 is the result of treatment with mutagen Ethyl Methane Sulfonate.  Based on the results of selection in vitro of potato plantlets M 1 Granola with suspense of Rs 9 × 10 10 CFU / ml, were can be induced E 9 three potato plantlets are rather susceptible to  Ralstonia solanacearum through the regenerated node and E 6 and E 8 treatment can be induced moderately resistant planlets against Ralstonia solanacearum. Keyword: EMS, Granola, Ralstonia solanacearum

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Abstrac Bacterial wilt disease caused by Ralstonia solanacearum is an important disease and could decreased yield up to 80%. Generally, bacterial disease is more difficult to control than other diseases. Moreover, there are no chemicals that can be used to control this pathogen. The approach through genetic improvement by using Ethyl Methane Sulfonate and in vitro selection are procesess to generate variants resistant against bacterial wilt on potato plants. Granola potato M 1 is the result of treatment with mutagen Ethyl Methane Sulfonate.  Based on the results of selection in vitro of potato plantlets M 1 Granola with suspense of Rs 9 × 10 10 CFU / ml, were can be induced E 9 three potato plantlets are rather susceptible to  Ralstonia solanacearum through the regenerated node and E 6 and E 8 treatment can be induced moderately resistant planlets against Ralstonia solanacearum. Keyword: EMS, Granola, Ralstonia solanacearum

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Available abstract

Abstrac Bacterial wilt disease caused by Ralstonia solanacearum is an important disease and could decreased yield up to 80%. Generally, bacterial disease is more difficult to control than other diseases. Moreover, there are no chemicals that can be used to control this pathogen. The approach through genetic improvement by using Ethyl Methane Sulfonate and in vitro selection are procesess to generate variants resistant against bacterial wilt on potato plants. Granola potato M 1 is the result of treatment with mutagen Ethyl Methane Sulfonate.  Based on the results of selection in vitro of potato plantlets M 1 Granola with suspense of Rs 9 × 10 10 CFU / ml, were can be induced E 9 three potato plantlets are rather susceptible to  Ralstonia solanacearum through the regenerated node and E 6 and E 8 treatment can be induced moderately resistant planlets against Ralstonia solanacearum. Keyword: EMS, Granola, Ralstonia solanacearum

Key concepts: Ralstonia solanacearum, Bacterial wilt, Ralstonia, Biology, Microbiology, Pathogen, Bacteria, Biotechnology

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