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2016•Unpublished venueRequires access

Rapid Diagnostic Test for the Diagnosis of Enteric Fever: A Cross-sectional Diagnostic Study

S Amudhavalli, C P Ramani, T Ravichandran, Arunagiri Kamalanathan, A Heber

Open publisher page 1 citations

Abstract

Results: Culture positive for Salmonella species was found to be 7.5%. Salmonella typhi was the predominant isolate (86%). S. paratyphi A (14%). Co-agglutination method showed positivity rate of 22.5%. Among the three types of samples, blood culture supernatant showed maximum positivity. 27% positivity was observed with the Widal test. PCR detected (10.8%) of cases. Conclusion: Rapid diagnostic test evaluations to date have used blood culture primarily as the reference standard for typhoid fever diagnosis. Culture provides definitive evidence of infection, but it fails to detect all cases, due to low numbers of the pathogen in the bloodstream and/or prior exposure to antibiotics. Thus, it is evident that PCR is highly specific, and co-agglutination test is highly sensitive for the diagnosis of enteric fever.

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What this paper is about

Results: Culture positive for Salmonella species was found to be 7.5%. Salmonella typhi was the predominant isolate (86%). S. paratyphi A (14%). Co-agglutination method showed positivity rate of 22.5%. Among the three types of samples, blood culture supernatant showed maximum positivity. 27% positivity was observed with the Widal test. PCR detected (10.8%) of cases. Conclusion: Rapid diagnostic test evaluations to date have used blood culture primarily as the reference standard for typhoid fever diagnosis. Culture provides definitive evidence of infection, but it fails to detect all cases, due to low numbers of the pathogen in the bloodstream and/or prior exposure to antibiotics. Thus, it is evident that PCR is highly specific, and co-agglutination test is highly sensitive for the diagnosis of enteric fever.

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Available abstract

Results: Culture positive for Salmonella species was found to be 7.5%. Salmonella typhi was the predominant isolate (86%). S. paratyphi A (14%). Co-agglutination method showed positivity rate of 22.5%. Among the three types of samples, blood culture supernatant showed maximum positivity. 27% positivity was observed with the Widal test. PCR detected (10.8%) of cases. Conclusion: Rapid diagnostic test evaluations to date have used blood culture primarily as the reference standard for typhoid fever diagnosis. Culture provides definitive evidence of infection, but it fails to detect all cases, due to low numbers of the pathogen in the bloodstream and/or prior exposure to antibiotics. Thus, it is evident that PCR is highly specific, and co-agglutination test is highly sensitive for the diagnosis of enteric fever.

Key concepts: Typhoid fever, Widal test, Enteric fever, Blood culture, Salmonella typhi, Salmonella, Medicine, Direct agglutination test

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