2017•Terapevticheskii arkhivOpen access
Absolute numbers of peripheral blood CD34+ hematopoietic stem cells prior to a leukapheresis procedure as a parameter predicting the efficiency of stem cell collection
Irina Vladimirovna Galtseva, Yulia O. Davydova, Т. В. Гапонова, Nikolay M Kapranov, Larisa A. Kuzmina, Vera V. Troitskaya, Elena Olegovna Gribanova, Sergey Kirillovich Kravchenko, Ya K Mangasarova, Eugene E. Zvonkov, Elena Nikolaevna Parovichnikova, Larisa Pavlovna Mendeleeva, Savchenko Vg
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Abstract
AIM: To identify a parameter predicting a collection of at least 2·106 CD34+ hematopoietic stem cells (HSC)/kg body weight per leukapheresis (LA) procedure. SUBJECTS AND METHODS: The investigation included 189 patients with hematological malignancies and 3 HSC donors, who underwent mobilization of stem cells with their subsequent collection by LA. Absolute numbers of peripheral blood leukocytes and CD34+ cells before a LA procedure, as well as a number of CD34+ cells/kg body weight (BW) in the LA product stored on the same day were determined in each patient (donor). RESULTS: There was no correlation between the number of leukocytes and that of stored CD34+ cells/kg BW. There was a close correlation between the count of peripheral blood CD34+ cells prior to LA and that of collected CD34+ cells calculated with reference to kg BW. CONCLUSION: The optimal absolute blood CD34+ cell count was estimated to 20 per µl, at which a LA procedure makes it possible to collect 2·106 or more CD34+ cells/kg BW.