益气活血软坚解毒方对荷H22 615小鼠肝癌细胞凋亡相关基因Fas、FasL、Bcl-2、Bax、P^53、NF-κB表达的影响

Objective: To study the effect of Yiqi, Huoxue, Ruanjian & Jiedu (YHRJ) Recipe on the expression of heaptocarcinoma cell, apoptosis regulate gene such as Fas, FasL, Bcl-2, Bax, P^53, NF-κB in mice with H22 615 Tumor. Methods: The mice were randomly divided into 6 groups: NS group, DDP group, YHRJ equivalent dosage group, YHRJ high dosage group, YHRJ equivalent dosage +DDP group, YHRJ high dosage +DDP group. Immunohistochemistry, situhybridization and RT-PCR were used to detect the key regulator genes of HCC apoptosis, Fas, FasL, Bcl-2, Bax, P^53, NF-κB protein and mRNA in mice with H22 615 tumor after 13 days treatment. Results: The immunohistochemistry results showed: compared with NS group, each adding medicine group could enhance the expression of Fas protein (P＜0.01) and YHRJ equivalent dosage +DDP group could decrease obviously the expression of FasL protein (P＜0.01). Compared with DDP group, Chinese medicines groups and Chinese medicines +DDP groups could decrease obviously the expression of FasL protein (P＜0.01). Compared with NS group, each adding medicine group could decrease obviously the expression of NF-κB protein (P＜0.05 or P＜0.01); Compared with DDP group, YHRJ equivalent dosage group, YHRJ high dosage group, YHRJ high dosage +DDP group could decrease obviously the expression of NF-κB protein (P＜0.01). Compared with NS group, each adding medicine group could decrease obviously the expression of mutated P^53 protein (P＜0.01); Compared with DDP group, Chinese medicines groups and Chinese medicines +DDP groups could decrease obviously the expression of mutated P^53 protein (P＜0.01). Compared with NS group, each adding medicine group could enhance obviously the expression of Bax protein (P＜0.01); Compared with DDP group, YHRJ high dosage group and Chinese medicines +DDP groups could enhance obviously the expression of Bax protein (P＜0.01). The situhybridization detect the expression of results NF-κB mRNA, the results showed: compared with NS group and DDP group, YHRJ equivalent dosage group and Chinese medicines +DDP groups could decrease obviously the expression of NF-κB mRNA (P＜0.01). The RT-PCR detect the expression of Bax, Bcl-2 mRNA, the results showed: compared with NS group and DDP group, YHRJ equivalent dosage group could enhance obviously the expression of Bax mRNA (P＜0.001). Compared with NS group, each adding medicine group could decrease obviously the expression of Bcl-2 mRNA (P＜0.05 or P＜0.001). Compared with DDP group, YHRJ high dosage group could decrease obviously the expression of Bcl-2 mRNA (P=0.01). Conclusion: YHRJ recipe could accelerate the apoptosis of HCC in mice with H22615 tumor, and enhance the gene expression of Fas and Bax, and decrease the expression of FasL and Bcl-2 (the restrain apoptosis genes), and decrease the expression of mutated P^53 (a apoptosis protection gene) protein and NF-κB gene. These roles are elements underlying to induce the HCC cells apoptosis.