2017•Current Analytical ChemistryOpen access

A Novel Method for the Analysis of Soluble Carbohydrates from Cistanche deserticola

Yuanheng Guo, Bing Zhao

Abstract

Objective: A comprehensive monosaccharide quantifying method was developed in this article. Monosaccharides (including glucose, arabinose, galacturonic acid, galactose, mannose, rhamnose and xylose) were analyzed by RP-HPLC-DAD method. Meanwhile, mannitol and fructose were calculated based on peak areas obtained by HPIEC-RID and corresponding data obtained by RP-HPLC-DAD. Monosaccharide compositions of CDSC were analyzed, nine kinds of monosacchardes (glucose, arabinose, galctose, galacturonic acid, mannose, rhamnose, xylose, fructose and mannitol) in were quantified. Keywords: Cistanche deserticola Y. C. Ma, soluble carbohydrates, monosaccharide compositions, high performance liquid chromatography, high-performance ion exclusion chromatography, herbal medicine.

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Available abstract

Key concepts: Monosaccharide, Rhamnose, Arabinose, Xylose, Mannitol, Fructose, Galactose, Chromatography