Detection of blaNDM -Metallo-β-Lactamase Genes in Klebsiella pneumonia Strains Isolated From Burn Patients in Baghdad Hospitals

From the period from March to August 2016,210 swabs were collected from the burn patients hospitalized indifferent hospitals in Baghdad City: Al-Karama Teaching Hospital,Special Burn Hospital,Central Teaching Laboratories,Child protection Teaching Hospital,Imam Ali Hospital. Out of 210 clinical isolates,42 (37.5 %) had been shown a single isolated of pathogenic bacteria K. pneumoniae and the others were belonged to other bacteria and mixed growth isolates. Identification of all isolates were carried out depending on macroscopic, microscopic characterizations,conventional biochemical tests and Api 20E system.Metallo-β lactamase (MBL) enzymes were screen by two phenotypic methods (Meropenem-EDTA double disks method and Modified Hodg test). Susceptibility testing were used with The following antibiotic disks:Imipenem, Meropenem, Ceftazidime, Cefotaxime, Pipracillin, Gentamicin, Amikacin andCiprofloxacin.The percentage of resistance isolates were as followed:Imipenem (21.42%), Meropenem (19.04%), Ceftazidime (69.04%),Cefotaxime (85.71%),Pipracillin (85.71%),Gentamicin (26.19%),Amikacin (19.04 %) andCiprofloxacin (59.52%).The percentage of the prevalence of blaNDM-1 and blaNDM-2 genes in K.pneumoniae isolates from burn patients in Baghdad hospitals were as followed:20 (100 %) forblaNDM-1 genes and 6 (30 %) for blaNDM-2 genes.