In vitro micropropagation and plants regeneration of Cowpea (Vigna unguiculata (L.) Walp) from cotyledonary node

Vigna unguiculata (L.) Walp plant was efficiently regenerated using cotyledonary nodes as explant. The explants were excised from 3 days old seedling grown in vitro on MS medium containing 3 mg/l BAP. They were cultured on MS medium containing BAP (2,5 mg/l) combinated with different dose of NAA (0, 0,1 and 0,5 mg/l) for shoot induction. Regeneration frequency varied with growth regulator combinations in the medium. A maximum number of 9,6 shoots per explant was recorded on MS medium containing 2.5 mg/l BAP. Increase in the ANA concentration of the culture medium decreased the number of shoots. Regenerated shoots were elongated on MS medium containing 1,25 mg/l BAP. The elongated shoots were rooted on MS rooting medium without hormone. The rooted plants were transferred to soil with a survival rate of 80%.