2006•The Proceedings of the Thermal Engineering ConferenceOpen access

A152 Study of the Effect of Antifreeze Protein on Ice Crystal Growth in One Direction

Yusuke Morikane, Rikiya Nakanishi, Yoshimiti Hagiwara

Abstract

We observe the gradual, one-directional freezing procedure of the aqueous solution of HPLC6, which is the major fraction of the antifreeze protein type I. The location of the protein is obtained from the fluorescence tagged to the protein. It is observed that the proteins slide on the interface. Also, long, narrow liquid regions are formed inside the ice from the depression parts due to the highly associated proteins. It is found from our supplementary computation that these liquid regions can be predicted.

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Key concepts: Antifreeze protein, Antifreeze, Ice crystals, Aqueous solution, Chemistry, Freezing-point depression, Protein crystallization, Crystallography