2017•Andalas University eThesis (Andalas University)Open access

ISOLASI DAN KARAKTERISASI BAKTERI PENGHASIL ENZIM PROTEASE NETRAL DARI DADIH

Annisa Aulia

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Abstract

Research isolation and characterization of bacteria producing an neutral protease enzyme of curds was carried out from October 2016 to January 2017 in the Laboratory of Microbiology Research Department of Biology, Faculty of Mathematics and Natural Sciences, University of Andalas, Padang and Testing Laboratory of Veterinary Investigation Centres Regional II Bukittinggi. This study used an experimental method, the data obtained from the study were analyzed and displayed in the form of images, tables and graphs descriptively. Isolation of bacteria producing protease enzyme neutral from buttermilk, found six isolates indicated as a producer of protease neutral by code isolates BKT-06, BKT-20, ALP-03, ALP-07, ALP-05, and ALP-21. The characters are all neutral protease-producing isolates from whey different from each other and biochemical tests showed that isolates ALP-03 is genus Bacillus sp. Isolate BKT-06, BKT-20, and ALP-07 is genus Lactobacillus sp. Isolate ALP-05 and ALP-21 is genus Staphylococcus sp. \n \nKey words : Isolation, characterization, neutral protease, curd.

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Research isolation and characterization of bacteria producing an neutral protease enzyme of curds was carried out from October 2016 to January 2017 in the Laboratory of Microbiology Research Department of Biology, Faculty of Mathematics and Natural Sciences, University of Andalas, Padang and Testing Laboratory of Veterinary Investigation Centres Regional II Bukittinggi. This study used an experimental method, the data obtained from the study were analyzed and displayed in the form of images, tables and graphs descriptively. Isolation of bacteria producing protease enzyme neutral from buttermilk, found six isolates indicated as a producer of protease neutral by code isolates BKT-06, BKT-20, ALP-03, ALP-07, ALP-05, and ALP-21. The characters are all neutral protease-producing isolates from whey different from each other and biochemical tests showed that isolates ALP-03 is genus Bacillus sp. Isolate BKT-06, BKT-20, and ALP-07 is genus Lactobacillus sp. Isolate ALP-05 and ALP-21 is genus Staphylococcus sp. \n \nKey words : Isolation, characterization, neutral protease, curd.

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Available abstract

Research isolation and characterization of bacteria producing an neutral protease enzyme of curds was carried out from October 2016 to January 2017 in the Laboratory of Microbiology Research Department of Biology, Faculty of Mathematics and Natural Sciences, University of Andalas, Padang and Testing Laboratory of Veterinary Investigation Centres Regional II Bukittinggi. This study used an experimental method, the data obtained from the study were analyzed and displayed in the form of images, tables and graphs descriptively. Isolation of bacteria producing protease enzyme neutral from buttermilk, found six isolates indicated as a producer of protease neutral by code isolates BKT-06, BKT-20, ALP-03, ALP-07, ALP-05, and ALP-21. The characters are all neutral protease-producing isolates from whey different from each other and biochemical tests showed that isolates ALP-03 is genus Bacillus sp. Isolate BKT-06, BKT-20, and ALP-07 is genus Lactobacillus sp. Isolate ALP-05 and ALP-21 is genus Staphylococcus sp. \n \nKey words : Isolation, characterization, neutral protease, curd.

Key concepts: Neutral protease, Protease, Isolation (microbiology), Microbiology, Bacteria, Enzyme, Biology, Food science

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