2017International Journal of Dairy TechnologyRequires access

Partial characterisation of a bacteriocin produced by Leuconostoc mesenteroides A11 and evaluation of bacteriocin production using whey as culture medium

Nelly K Okuda, Fabrício Luiz Tulini, Lizziane Kretli Winkelströter, Elaine Cristina Pereira De Martinis

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Abstract

This study aimed to partially purify and characterise the bacteriocin produced by Leuconostoc mesenteroides A11 and to evaluate the bacteriocin production in whey at different concentrations and temperatures (30 and 25 °C). The partially purified bacteriocin was obtained by chromatography and analysed by electrophoresis. Thus, the molecular weight of the bacteriocin was estimated by electrophoresis, ranging from 3.5 to 8.5 kDa. Leu conostoc mesenteroides A11 produced bacteriocins in all concentrations of whey and temperatures evaluated, indicating that it would be feasible to employ low whey concentrations and room temperature for the production of bacteriocins, thereby reducing processing costs.

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What this paper is about

This study aimed to partially purify and characterise the bacteriocin produced by Leuconostoc mesenteroides A11 and to evaluate the bacteriocin production in whey at different concentrations and temperatures (30 and 25 °C). The partially purified bacteriocin was obtained by chromatography and analysed by electrophoresis. Thus, the molecular weight of the bacteriocin was estimated by electrophoresis, ranging from 3.5 to 8.5 kDa. Leu conostoc mesenteroides A11 produced bacteriocins in all concentrations of whey and temperatures evaluated, indicating that it would be feasible to employ low whey concentrations and room temperature for the production of bacteriocins, thereby reducing processing costs.

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Available abstract

This study aimed to partially purify and characterise the bacteriocin produced by Leuconostoc mesenteroides A11 and to evaluate the bacteriocin production in whey at different concentrations and temperatures (30 and 25 °C). The partially purified bacteriocin was obtained by chromatography and analysed by electrophoresis. Thus, the molecular weight of the bacteriocin was estimated by electrophoresis, ranging from 3.5 to 8.5 kDa. Leu conostoc mesenteroides A11 produced bacteriocins in all concentrations of whey and temperatures evaluated, indicating that it would be feasible to employ low whey concentrations and room temperature for the production of bacteriocins, thereby reducing processing costs.

Key concepts: Bacteriocin, Leuconostoc mesenteroides, Food science, Chemistry, Leuconostoc, Gel electrophoresis, Bacteria, Chromatography

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