2000•Chinese Journal of Appplied Environmental BiologyRequires access
GENETIC DIVERSITY DETECTED BY RAPD IN FIVE VELVET-DEER BREEDS OR STRAINS OF CHINA
Heping Li, Shi ShouKun, Sheng Li
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Abstract
RAPD analysis was used to estimate genetic diversity and relationship in 75 individuals which belong to five deer breeds or strains. 41 primers were used, among which 40 generated polymorphic loci. A total of 359 bands were scored, of which 259 (65.6%) were polymorphic. Shannon index was used to estimate the genetic diversity and the proportion of genetic variation within and between populations. The genetic distance among individuals was obtained by proportion of shared fragments. Dendrogram was generated by UPGMA and reflected the genetic relationship among the five deer breeds or strains. The results indicated that the genetic diversity of Shuangyang was the lowest in the five populations. About 54% variation was detected within populations and 46% among populations. The genetic diversity index of five breeds and strains was 1.124 9 in Xifeng, 1.051 7 in Changbaishan, 0.990 8 in Wulanba, 0.974 8 in Qingyuan and 0.766 2 in Shuangyang. Fig 1, Tab 3, Ref 14