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Chromatographic analysis (TLC) of uracil arabinoside, cytosine arabinoside, uracil and cytosine in human plasma.

Beata Paw, Genowefa Misztal

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Abstract

Cytosine arabinoside, uracil arabinoside, cytosine and uracil after solid-phase extraction from plasma with minicolumns Adsorbex were separated by TLC on silica gel 60 GF254 by ascending and horizontal technique using suitable mobile phases. The substances were identified by UV irradiation at 254 nm and by spraying of 1% KMnO4 solution (up to the amount 500 ng analysed substances).

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Cytosine arabinoside, uracil arabinoside, cytosine and uracil after solid-phase extraction from plasma with minicolumns Adsorbex were separated by TLC on silica gel 60 GF254 by ascending and horizontal technique using suitable mobile phases. The substances were identified by UV irradiation at 254 nm and by spraying of 1% KMnO4 solution (up to the amount 500 ng analysed substances).

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Available abstract

Cytosine arabinoside, uracil arabinoside, cytosine and uracil after solid-phase extraction from plasma with minicolumns Adsorbex were separated by TLC on silica gel 60 GF254 by ascending and horizontal technique using suitable mobile phases. The substances were identified by UV irradiation at 254 nm and by spraying of 1% KMnO4 solution (up to the amount 500 ng analysed substances).

Key concepts: Cytosine, Uracil, Chemistry, Chromatography, DNA, Silica gel, Biochemistry

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Chromatographic analysis (TLC) of uracil arabinoside, cytosine arabinoside, uracil and cytosine in human plasma. — Research Paper | ScholarLens