Effects of different doses of manganese on lead poisoning in the kidney of adult male mice

Introduction: Lead is one the heavy metals that cause negative effects on the body systems. However, there is little data about the effect of antioxidants on kidney damage induced by the lead. Therefore, the aim of the present study was to evaluate the effects of different doses of manganese on biochemical and histopathological parameters of kidney in mice.\n\nMaterials and Methods: In this study, 48 adult Balb/C male mice were randomly divided into six groups. Control group did not receive any injection. Second group received 60 mg/kg lead acetate intraperitoneally. Third to sixth groups were injected 60 mg/kg lead acetate and 2.5-5-10-20 mg/kg manganese intraperitoneally, respectively. After 14 days, slides from kidney of mice prepared and the volume of kidney and volume and number of glomeruli were measured by stereological method. Besides, levels of serum urea and creatine were measured.\n\nResults: Evaluation of the kidney tissue in lead poisoning group showed collapse and glomerulosclerosis as well as tubular vacuolization and necrosis. In the 2.5 mg/kg manganese-treated group less tissue damage occurred. A significant increase in volume of kidney was observed in 2.5, 5 mg/kg manganese-treated and lead poisoning groups (P <0.05). The number of glumeruli in 5, 10 and 20 mg treated manganese groups showed significant increase in compare to the lead poisoning group. Urea level in all groups under treatment with manganese (P = 0.000) and creatinine level in 2.5 mg treated manganese group were significant reduced in compare to those in the lead poisoning group (P = 0.01).\n\nConclusion: Treatment with 2.5 mg/kg and 5 mg/kg of manganese after injection of lead acetate for 14 days caused improvement in histopathology and biochemical signs of kidney tissue damage in mice.