Construction of A pUC19-T Vector Based on Xcm I

[Objective] The paper aimed to construct a new T vector based on plasmid pUC19 digested with Xcm I. [Method] Two complementary oligonucleotide chains containing two Xcm I sites were synthesized. After denaturation and renaturation, the adaptor was cloned into plasmid pUC19 between the Hind III and BamH I sites. The new plasmid, pUC19-HB-T vector, was digested with Xcm I to derive a T-vector with 3′ end overhanging a T base. [Result] The constructed pUC19-HB-T vector was efficient in cloning PCR products, with an efficiency of 95% at least. [Conclusion] A new Xcm I-based pUC19-HB-T vector was constructed, which could be applied to cloning of PCR products and other microbiology operations.