Antioxidant activities from various bulbs extracts of three kinds allium using DPPH, ABTS assays and correlation with total phenolic, flavonoid, carotenoid content
Irda Fidrianny, Listya Permatasari, Komar Ruslan Wirasutisna
Abstract
Irda Fidrianny, Listya Permatasari, Komar Ruslan Wirasutisna
Abstract
The antioxidant activities of various bulbs extracts of three kinds Allium were conducted by DPPH (2.2-diphenyl-1-picrylhydrazyl) and ABTS (2-2’-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) assays. Sample O2 (ethyl acetate extract of onion bulbs) had the highest DPPH scavenging capacity (45.86 %.) and the highest ABTS capacity (49.03 %). Sample RO2 (ethyl acetate extract of sample red onion) contained the highest total flavonoid (89.3 g QE/100 g) and the highest phenolic contents (40.75 g GAE/100 g), while the highest carotenoid 7.4 g BET/100 g was given by RO1 (n-hexane extract of sample red onion). Phenolic and flavonoid were the major contributors to antioxidant activity in onion and red onion bulbs. The total carotenoid of three Allium bulbs extracts had no significant correlation with DPPH and ABTS scavenging activities. There were positively high correlation between ABTS and DPPH scavenging capacities for sample O and RO, but no correlation for sample G (garlic).
OpenAlex reports 15 citations for this work. Citation counts describe recorded attention and do not establish research quality.
A contribution statement is not available in the OpenAlex record.
Method details are not available in the OpenAlex metadata.
Findings are not separately available in the OpenAlex metadata.
Limitations are not available in the OpenAlex metadata.
Application details are not available in the OpenAlex metadata.
The antioxidant activities of various bulbs extracts of three kinds Allium were conducted by DPPH (2.2-diphenyl-1-picrylhydrazyl) and ABTS (2-2’-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) assays. Sample O2 (ethyl acetate extract of onion bulbs) had the highest DPPH scavenging capacity (45.86 %.) and the highest ABTS capacity (49.03 %). Sample RO2 (ethyl acetate extract of sample red onion) contained the highest total flavonoid (89.3 g QE/100 g) and the highest phenolic contents (40.75 g GAE/100 g), while the highest carotenoid 7.4 g BET/100 g was given by RO1 (n-hexane extract of sample red onion). Phenolic and flavonoid were the major contributors to antioxidant activity in onion and red onion bulbs. The total carotenoid of three Allium bulbs extracts had no significant correlation with DPPH and ABTS scavenging activities. There were positively high correlation between ABTS and DPPH scavenging capacities for sample O and RO, but no correlation for sample G (garlic).
Key concepts: DPPH, ABTS, Chemistry, Flavonoid, Allium, Food science, Ethyl acetate, Carotenoid