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2017•Analytical ChemistryRequires access

Bioluminescence Sensing of γ-Glutamyltranspeptidase Activity In Vitro and In Vivo

Zijuan Hai, Jingjing Wu, Lin Wang, Jiacheng Xu, Huafeng Zhang, Gaolin Liang

Open publisher page 52 citations

Abstract

γ-Glutamyltranspeptidase (GGT) is an important tumor biomarker but using a bioluminescence (BL) probe to real time monitor its activity has not been reported. Herein, we rationally designed two GGT-cleavable BL probes Glu-AmLH 2 ( 1 ) and Glu- p -aminobenzyloxycarbonyl-AmLH 2 ( 2 ), and successfully applied them for sensing GGT activity with high sensitivity and excellent selectivity both in vitro and in vivo. The results indicated that, although 2 had lower background BL signal than 1, GGT had higher catalytic efficiency for 1 than 2, and 1 was superior to 2 for sensing GGT activity in living cells and tumors. We envision that our probe 1 could be widely applied for the diagnosis of important GGT-related diseases in animal models in the near future.

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What this paper is about

γ-Glutamyltranspeptidase (GGT) is an important tumor biomarker but using a bioluminescence (BL) probe to real time monitor its activity has not been reported. Herein, we rationally designed two GGT-cleavable BL probes Glu-AmLH 2 ( 1 ) and Glu- p -aminobenzyloxycarbonyl-AmLH 2 ( 2 ), and successfully applied them for sensing GGT activity with high sensitivity and excellent selectivity both in vitro and in vivo. The results indicated that, although 2 had lower background BL signal than 1, GGT had higher catalytic efficiency for 1 than 2, and 1 was superior to 2 for sensing GGT activity in living cells and tumors. We envision that our probe 1 could be widely applied for the diagnosis of important GGT-related diseases in animal models in the near future.

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OpenAlex reports 52 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

γ-Glutamyltranspeptidase (GGT) is an important tumor biomarker but using a bioluminescence (BL) probe to real time monitor its activity has not been reported. Herein, we rationally designed two GGT-cleavable BL probes Glu-AmLH 2 ( 1 ) and Glu- p -aminobenzyloxycarbonyl-AmLH 2 ( 2 ), and successfully applied them for sensing GGT activity with high sensitivity and excellent selectivity both in vitro and in vivo. The results indicated that, although 2 had lower background BL signal than 1, GGT had higher catalytic efficiency for 1 than 2, and 1 was superior to 2 for sensing GGT activity in living cells and tumors. We envision that our probe 1 could be widely applied for the diagnosis of important GGT-related diseases in animal models in the near future.

Key concepts: Chemistry, In vivo, Bioluminescence, In vitro, Biomarker, Selectivity, Biochemistry, Molecular biology

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