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2014•Journal of chemical and pharmaceutical researchRequires access

Analysis of 50% acetochlor and clomazone EC by HPLC.

Fan Xiaozhen, Xiaoyun Liu, Cuihua Zhang, Xu Bao-jun

Open publisher page 2 citations

Abstract

RP-HPLC was adopted for the separation and determination of acetochlor and clomazone on the BDS Hypersil C18 column (5μm, 250mm×4.6mm), acetonitrile-water (volume ratio 60:40) as the mobile phase, and the UV detector with wavelength 210nm. The results show that the retention times of acetochlor and clomazone are 9.065min and 5.598min respectivelyi¼Œand the linear correlation coefficients for both acetochlor and clomazone are 0.9998, the relative standard deviations are 1.25% and 1.60% respectively, the average recovery rates are 99.14% and 98.15%. This method is simple, accurate and highly selective.

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What this paper is about

RP-HPLC was adopted for the separation and determination of acetochlor and clomazone on the BDS Hypersil C18 column (5μm, 250mm×4.6mm), acetonitrile-water (volume ratio 60:40) as the mobile phase, and the UV detector with wavelength 210nm. The results show that the retention times of acetochlor and clomazone are 9.065min and 5.598min respectivelyi¼Œand the linear correlation coefficients for both acetochlor and clomazone are 0.9998, the relative standard deviations are 1.25% and 1.60% respectively, the average recovery rates are 99.14% and 98.15%. This method is simple, accurate and highly selective.

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Available abstract

RP-HPLC was adopted for the separation and determination of acetochlor and clomazone on the BDS Hypersil C18 column (5μm, 250mm×4.6mm), acetonitrile-water (volume ratio 60:40) as the mobile phase, and the UV detector with wavelength 210nm. The results show that the retention times of acetochlor and clomazone are 9.065min and 5.598min respectivelyi¼Œand the linear correlation coefficients for both acetochlor and clomazone are 0.9998, the relative standard deviations are 1.25% and 1.60% respectively, the average recovery rates are 99.14% and 98.15%. This method is simple, accurate and highly selective.

Key concepts: Clomazone, Acetochlor, Chromatography, High-performance liquid chromatography, Chemistry, Acetonitrile, Linear correlation, Mathematics

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