Estimation of Delafloxacin Using Derivative Spectrophotometry and Area Under Curve in Bulk Material and in Laboratory Mixture

Simple, specific, rapid and accurate UV-spectrophotometric methods have been developed using a solvent acetonitrile (50 %) to determine delafloxacin in bulk material and in laboratory mixture. “Method A” is zero order derivative UV- spectrophotometry using absorbance, “Method B” is zero order derivative UV-spectrophotometry using Area Under Curve (AUC) technique, “Method C” is first order derivative UV-spectrophotometry using amplitude, “Method D” is First Order Derivative UV-spectrophotometry- AUC,“Method E” is Second Order Derivative UV-spectrophotometry using amplitude and “Method F” is second order derivative UV- spectrophotometry using (AUC) technique. The developed methods have shown excellent results in terms of linearity and range, accuracy, precision and Limit of Detection (LOD) and Limit of Quantification (LOQ). In all Methods, delafloxacin obeyed linearity in the concentration range of 2 – 12 μg/mL with (r2 > 0.999). All these methods were applied for estimation of delafloxacin in laboratory mixture. All the above mentioned methods were validated considering linearity and range, accuracy, precision, ruggedness and sensitivity.